Curcumin combining with si-MALAT1 inhibits the invasion and migration of colon cancer SW480 cells

To study the effect of small interfering RNA targeting metastasis-associated lung adenocarcinoma transcript1 (si-MALAT1) combining with curcumin on the invasion and migration abilities of human colon cancer SW480 cells, and to explore the involved molecular mechanism. The recombinant lentiviral vector expressing si-MALAT1 was constructed, and its titer was determined by gradient dilution method. The colon cancer SW480 cells with stable expression of si-MALAT1 was established, followed by treatment with curcumin at different concentrations. The effect of curcumin or si-MALAT1 alone and the combination of the two on the cell activity was detected by MTT assay. The cell invasion and migration abilities were detected by transwell and scratch-wound assay. The relative expression level of MALAT1 was detected by RT-qPCR. The protein expression was determined by Western blot analysis. The IC50 of curcumin alone was 77.69 mmol/L, which was 51.17 mol/L when combined with curcumin and random sequence. The IC50 of curcumin was 30.02 mmol/L when combined with si-MALAT1. The increased susceptibility multiples was 2.58. The wound healing rates were 30.9% and 67.5% after treatment with si-MALAT1 combined with curcumin for 24 hrs and 48 hrs, respectively. The numbers of invasion cells were 200±12, 162±13, 66±8, 53±4 and 16±3 after treatment with si-MALAT1 combined with curcumin for 48 hrs. The relative expression level of lncRNA-MALAT1 in the curcumin group was 68%, and the relative expression level of lncRNA-MALAT1 in si-MALAT1group was 56%, and that for the combination treatment group was about 21%. The protein expression levels of β- catenin, c-myc and cyclinD1 were significantly down-regulated upon treatment with certain concentration of si-MALAT1 alone or combined with curcumin.si-MALAT1 could significantly inhibit the invasion and migration of SW480 cells by enhancing the sensitivity of SW480 cells to curcumin. The mechanism involved mignt be related to the down-regulation of β-catenin, c-myc and cyclinD1 proteins.

Application analysis of time-resolved fluorescence immunoassay technology in syphilis specific antibody screening / 国际检验医学杂志

Objective To investigate the application of time‐resolved fluorescence immunoassay (TRFIA) in the detection of specific antibody of syphilis .Methods Specific antibody of syphilis was detected in serum samples of 240 cases of syphilis and 150 healthy subjects by TRFIA ,Treponemal pallidum particle agglutination(TPPA) and Treponemal pallidum enzyme linked immu‐nosorbent assay(TP‐ELISA) .The sensitivity ,specificity and positivity of these three methods were compared .Results The sensi‐tivity of TRFIA ,TP‐ELISA ,TPPA were 100 .00% ,98 .75% and 97 .92% ,without significantly differences(P>0 .05) ,and the spe‐cificity were 99 .33% ,98 .67% and 100 .00% .The false positive rate of TRFIA was 0 .67% ,and the false negative rate was 0 .00% . The false positive rate of TP‐ELISA was 1 .33% ,and the false negative rate was 1 .25% .False positive rate and false negative rate of TRFIA were lower than TP‐ELISA(P<0 .05) .Conclusion TRFIA could be with high sensitivity and specificity in syphilis spe‐cific antibody test ,and could be used for routine screening of syphilis specific antibody .